Development of 11 Polymorphic Microsatellite Loci from Primula amthystina Franchet. (Primulaceae)
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چکیده
منابع مشابه
Development of 12 polymorphic microsatellite loci in the high alpine perennial Primula halleri (Primulaceae)1
UNLABELLED PREMISE OF THE STUDY Primula halleri is a high alpine endemic species with a patchy distribution in Central and Eastern European mountains. Little is known about the structure of genetic variation and mating system in populations of this species. • METHODS AND RESULTS We report on the development of 12 novel, polymorphic microsatellite loci for P. halleri. Tests for amplificatio...
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PREMISE OF THE STUDY Microsatellite markers from Primula sikkimensis (Primulaceae) were developed for testing deep lineage divergence and speciation events. METHODS AND RESULTS A total of 3112 microsatellites were identified from 61,755 unique reads though 454 pyrosequencing technology. Twenty-nine microsatellite loci were selected for PCR amplification and polymorphic analyses. Among the 29 ...
متن کاملCharacterization of Novel Microsatellite Loci for Primula poissonii (Primulaceae) Using High-Throughput Sequencing Technology.
Primula poissonii (Primulaceae) is a perennial herb, widely distributed in the Hengduan Mountain region of Southwest China. In this study, Roche 454 pyrosequencing was used to isolate microsatellite markers. A total of 4528 unique sequences were identified from 68,070 unique reads. Of these, eighty-seven microsatellite loci were screened for utility using two criteria: successful PCR amplificat...
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UNLABELLED PREMISE OF THE STUDY We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. • METHODS AND RESULTS DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pai...
متن کاملHigh-throughput microsatellite marker development for the distylous herb Primula mistassinica (Primulaceae)1
UNLABELLED PREMISE OF THE STUDY Twelve microsatellite markers were developed for Primula mistassinica, a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. • METHODS AND RESULTS We used Roche/454 high-throughput technology to sequence micro...
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ژورنال
عنوان ژورنال: HortScience
سال: 2010
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.45.1.148